Inauguration STED nanoscope

(12-10-2021)

We cordially invite you on the festive inauguration of a STED nanoscope, part of the Centre for Advanced Light Microscopy.

When: 17 November 2021, 12.00h

Where: Classroom 0.3, Blok A, Ottergemsesteenweg 460, Campus Heymans, 9000 Gent

Program:

12.00 – 12.05: Welcome by Prof. Kevin Braeckmans

12.05 – 12.15: Introduction to the Centre for Advanced Light Microscopy by dr. Herlinde De Keersmaecker

12.15 – 12.45: Lecture by Martin Meschkat, application specialist Abberior Instruments

12.45 – 14.00: Sandwich lunch with possibility to take a look at the STED nanoscope (10 min)

14.00 – 18.00: '1 hour' demonstrations

During this program we offer you a sandwich lunch.

Register here

What is STED nanoscopy?

Since the introduction of confocal microscopy in the ‘80s, advanced light microscopy became indispensable in life science research. The wish to ever see more details has driven new developments in light microscopy. Although, the laws of physics predicted a fundamental limit to the maximal achievable optical resolution, Dr. Stephan Hell was one of the first to realize that this limit might not be as unsurpassable as it was believed to be. Thanks to advances in fluorescent labels and laser technology, he and others demonstrated that it was possible to visualize beyond the resolution limit of light with a diverse set of technologies that are now referred to as ‘super resolution microscopy’ or ‘nanoscopy’, for which the Nobel Prize in Chemistry was awarded in 2014.

Perhaps the most applicable form of 'nanoscopy' is Stimulated Emission Depletion (STED) nanoscopy which makes use of two coinciding laser beams that are scanned across the sample to generate an image. The first laser beam serves, as with confocal microscopy, to excite the fluorophores, while the second laser beam has a ‘donut’ shape which suppresses fluorescence except in the centre. This generates an effective resolution of 30 - 80 nm, similar to the size of e.g. a ribosome.

Thanks to financing  by the Midscale Infrastructure grant of FWO, the Centre for Advanced Light Microscopy has been able to purchase an unique state-of-the-art STED nanoscope which will by festively inaugurated on this occasion. Via the Centre for Advanced Light Microscopy this fascinating technology will be available for the research community at Ghent university and beyond.