Confocal & multi-photon microscopes

Overview of confocal microscopes available at the expertise centre and their unique features:

Nikon A1R HD confocal & multiphoton microscope

4 lasers (405 nm, 488 nm, 561 nm, 640 nm)
Galvoscanner and resonantscanner
Perfect Focus System
4 detection channels (2 GaASP and 2PMT detectors) with choice of different emission filters
32-channel spectral detector unit
Incubator with temperature regulation at the microscope level and CO₂ at the cell culture level
Multi-photon laser (Mai Tai TI:SAPPHIRE laser, 690-1040 nm)
Non-descanned detectors
Objective lenses:
- 60X water objective with automated correction ring (SR plan apo IR 60X WI, NA 1.3, WD 180µm)
- 60X oil (plan apo λ 60X oil, NA 1.4, WD 130 µm)
- 40X air (plan apo λ 40X, NA 0.9, WD 250 µm)
- 20X air (plan apo VC 20X, NA 0.8, WD 1000 µm)

Faculty of Pharmaceutical Sciences,

Department of Pharmaceutics,

Ottergemsesteenweg 460,

B-9000, Ghent

4 lasers with in total 7 laser lines (405 nm, 457 nm, 476 nm, 488 nm, 514 nm, 561 nm, 639 nm)
Galvoscanner and resonantscanner. Simultaneous use possible for photo-stimulation experiments (FRAP, FRET, ...)
Piezo stage for fast and precise scanning in the z direction
Perfect Focus System
Simultaneous 4-channel PMT detection with choice of different emission filters
32-channel spectral PMT detector, useful for spectral imaging
2 separated lasers for Controlled Light Exposure Microscopy
Incubator with temperature regulation at the microscope level and CO₂ at the cell culture level

Faculty Bioscience Engineering,

Vakgroep Biotechnologie, BW25

Campus Proeftuin, Gebouw N1, Lokaal 36.01.100.030

B-9000, Ghent

Faculty of Pharmaceutical Sciences,

Department of Pharmaceutics,

Ottergemsesteenweg 460,

B-9000, Ghent

White light laser (470 – 670 nm), up to 8 freely tunable laser lines and 2 UV Laser lines (355 nm, 405 nm)
2 HyD (sensitive detection) and 2 PMT detectors
Flexible tuning of emission bands via acousto-optical beam splitter (AOBS)
Possibility of spectral imaging, (UV) photo-stimulation and resonant scanner
Live cell imaging: Temperature and CO₂ incubator

Faculty of Medicine and Health Sciences,

Department of Basic Medical Sciences,

C. Heymanslaan 10, Blok B,

B-9000, Ghent