Guidelines PCR analysis
Guidelines
- For starters, take a hands-on course on PCR and pipetting.
- Think about your experiment, plan and consult to avoid unnecessary or unusable runs.
- Buy what you need and do it together.
- Use best practice guidelines and relevant controls.
- Avoid optimization runs with relentless in-silico characterization of primers, probes and normalization genes.
- Do not store your work stocks of primers and probes separately, but together in 1 ep.
- Work in small volumes (10µl for normal reaction, 20µl for cloning), consider 384 well plates.
- Avoid duplicates as much as possible.
- Automation can reduce consumption of consumables.
- Set room temperature for your last cycle.
Course
Organise a hands-on course. Information is following.